72P Correlation of serum cytokine patterns and clinicopathological factors in breast carcinoma patients

Recent evidence support that the immune system has both positive and negative effects on tumorigenesis. Systemic inflammation been linked to aggressive tumor growth, metastasis, drug resistance in breast cancer (BC) patients. Therefore, serum cytokines (SC) may represent an exciting biomarker monitoring of BC pathogenesis. Pretreatment from 204 patients (23% Luminal A, 23.6% B/HER2-, 22.5% B/HER2+, 10.3% HER2-enriched, 20.6% Triple-Negative/Basal-like -TNBL-) 50 healthy donors was collected. Measurement 62 SC performed using LEGENDplex immunoassay. Fluorescence intensity quantified by flow cytometry. The results were correlated with age, size grade, vascular invasion, necrosis, phenotype, tumor-infiltrating lymphocytes, lymph node status, Ki67. Statistical analysis done Student’s t-test Mann–Whitney U test. showed higher levels SCF, MIP3α, EPO 4-1BB but lower IL-2RA, IL-8, IL-12p40, IL-18, IL-23, IL-27, PDGF-AA, Galectin 9, PDGF-BB, B7.2, MIP1β PD1, compared group. Furthermore, younger age (p≤0.041), contrast MCP1, (p≤0.003). IL-12p40 IFNγ elevated cases grade I tumors (p≤0.05) and, addition IL-11 IL-27 low Ki67 (p≤0.030). Moreover, IL-23 associated nodes (p≤0.031). In tumors, we detected high IL-15, (p≤0.048). However, only PDGF-BB seen non-Luminal (p=0.040). mainly while PDGF-AA B/HER2-. Likewise, MIP1β, B/HER2+ serum, whereas Galectin9, 4-1BB, PD1 found TNBL, no specific profile for HER2-enriched. Our profiles among phenotypes. involvement especially A.